Tanshinone IIA and Cryptotanshinone Counteract Inflammation by Regulating Gene and miRNA Expression in Human SGBS Adipocytes

Inflammation of the adipose tissue contributes to onset and progression several chronic obesity-related diseases. The two most important lipophilic diterpenoid compounds found in root Salvia milthorrhiza Bunge (also called Danshen), tanshinone IIA (TIIA) cryptotanshinone (CRY), have many favorable pharmacological effects. However, their roles obesity-associated adipocyte inflammation related sub-networks not been fully elucidated. In present study, we investigated gene, miRNAs protein expression profile prototypical dysfunction markers inflamed human adipocytes treated with TIIA CRY. results showed that CRY prevented tumor necrosis factor (TNF)-α induced inflammatory response adipocytes, by counter-regulating pattern secreted cytokines/chemokines associated (CCL2/MCP-1, CXCL10/IP-10, CCL5/RANTES, CXCL1/GRO-α, IL-6, IL-8, MIF PAI-1/Serpin E1) via modulation gene (as demonstrated for CCL2/MCP-1, IL-8), as well miRNA (miR-126-3p, miR-223-3p, miR-124-3p, miR-155-5p, miR-132-3p), attenuating monocyte recruitment. This is first demonstration a beneficial effect on obesity development complications, offering new outlook prevention and/or treatment metabolic